Protective effects of pentoxifylline on T-cell viability under inflammatory conditionsopen access
- Authors
- Park, Sung-Joon; Choi, Sung-Hyuk; Cho, Young-Duck; Kim, Jung-Youn; Cho, Han-Jin; Kim, Kyung-Hwan; Kim, Won-Young
- Issue Date
- Aug-2022
- Publisher
- Biolife
- Keywords
- Trauma; pentoxifylline; lymphocyte; toll-like receptor; interleukin 2
- Citation
- European Journal of Inflammation, v.20
- Indexed
- SCIE
SCOPUS
- Journal Title
- European Journal of Inflammation
- Volume
- 20
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2021.sw.kumedicine/61452
- DOI
- 10.1177/1721727X221120753
- ISSN
- 1721-727X
2058-7392
- Abstract
- Introduction: Pentoxifylline (PTX) reduces the levels of pro-inflammatory cytokines; however, its effects on immune system is not well understood. The aim of this study was to investigate the effect of PTX on T cells under inflammatory conditions in co-culture with THP-1-derived macrophages.
Methods: Toll-like receptor 4 (TLR4) and macrophage migration inhibitory factor (MIF) levels were measured after addition of PTX to lipopolysaccharide (LPS)-stimulated differentiated THP-1 cells. T cell viability and MIF levels were measured after PTX was added to prostaglandin E2 (PGE2)-stimulated Jurkat T-cell leukemia line. Co-culture was conducted to determine the effect of LPS-stimulated differentiated THP-1 cells that are affected by PTX on Jurkat cells. To prevent the direct effects of LPS and PTX on Jurkat cells, LPS and PTX were washed from THP-1 cells before co-culture. T cell viability and interleukin-2 (IL-2) levels were determined in Jurkat cells.
Results: Increase in the MIF concentration and TLR4 expression level in differentiated THP-1 cells stimulated with LPS were reversed after PTX addition. However, PTX did not improve T cell viability in PGE2–stimulated Jurkat cells. Co-culturing Jurkat cell and LPS-stimulated differentiated THP-1 cells resulted in a decreased viability of T cells. The addition of PTX restored T cell viability to normal control levels and IL-2 expression level in Jurkat cells.
Conclusion: LPS-stimulated THP-1-derived macrophages reduced the T cell viability under inflammation. However, PTX restored T cells viability and IL-2 back to normal levels. Therefore, the immunomodulatory action of PTX may be mediated by macrophage-T cell interactions.
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- Appears in
Collections - 2. Clinical Science > Department of Emergency Medicine > 1. Journal Articles
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