Orthogonally-tunable and ER-targeting fluorophores detect avian influenza virus early infectionopen access
- Authors
- Kang, Taewon; Haque, Md Mamunul; Lee, Boran; Hong, Kyung Tae; Hong, Seong Cheol; Kim, Younghun; Lee, Jesang; Lee, Jun-Seok; Lee, Dongwhan
- Issue Date
- Oct-2022
- Publisher
- Nature Publishing Group
- Citation
- Nature Communications, v.13, no.1
- Indexed
- SCIE
SCOPUS
- Journal Title
- Nature Communications
- Volume
- 13
- Number
- 1
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2021.sw.kumedicine/61644
- DOI
- 10.1038/s41467-022-33586-1
- ISSN
- 2041-1723
2041-1723
- Abstract
- Cell-based assays can monitor virus infection at a single-cell level with high sensitivity and cost-efficiency. For this purpose, it is crucial to develop molecular probes that respond selectively to physiological changes in live cells. We report stimuli-responsive light-emitters built on a T-shaped benzimidazole platform, and consecutive borylation reactions to produce a library of homologs displaying systematic changes in fluorescence quantum yield and environmental sensitivity. We find that certain fluorophores localize selectively at the endoplasmic reticulum, and interact with proteins involved in the stress signaling pathways. Notably, the mono-borylated compound responds selectively to the stress conditions by enhancing fluorescence, and detects avian influenza virus infection at the single-cell level. Our findings demonstrate the unprecedented practical utility of the stress-responsive molecular probes to differentiate cellular states for early diagnosis.
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- Appears in
Collections - 1. Basic Science > Department of Pharmacology > 1. Journal Articles
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