In Vitro Anti-Inflammatory and Antioxidant Activities of pH-Responsive Resveratrol-Urocanic Acid Nano-Assembliesopen access
- Authors
- Song, Heegyeong; Kang, Seok; Yu, Ying; Jung, Sung Yun; Park, Kyeongsoon; Kim, Sang-Min; Kim, HaK-Jun; Kim, Jae Gyoon; Kim, Sung Eun
- Issue Date
- Feb-2023
- Publisher
- Multidisciplinary Digital Publishing Institute (MDPI)
- Keywords
- resveratrol; urocanic acid; pH responsive; anti-inflammatory; antioxidant; nanoparticles
- Citation
- International Journal of Molecular Sciences, v.24, no.4
- Indexed
- SCIE
SCOPUS
- Journal Title
- International Journal of Molecular Sciences
- Volume
- 24
- Number
- 4
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2021.sw.kumedicine/62567
- DOI
- 10.3390/ijms24043843
- ISSN
- 1661-6596
1422-0067
- Abstract
- Inflammatory environments provide vital biochemical stimuli (i.e., oxidative stress, pH, and enzymes) for triggered drug delivery in a controlled manner. Inflammation alters the local pH within the affected tissues. As a result, pH-sensitive nanomaterials can be used to effectively target drugs to the site of inflammation. Herein, we designed pH-sensitive nanoparticles in which resveratrol (an anti-inflammatory and antioxidant compound (RES)) and urocanic acid (UA) were complexed with a pH-sensitive moiety using an emulsion method. These RES-UA NPs were characterized by transmission electron microscopy, dynamic light scattering, zeta potential, and FT-IR spectroscopy. The anti-inflammatory and antioxidant activities of the RES-UA NPs were assessed in RAW 264.7 macrophages. The NPs were circular in shape and ranged in size from 106 to 180 nm. The RES-UA NPs suppressed the mRNA expression of the pro-inflammatory molecules inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), interleukin-1 beta (IL-1 beta), and tumor necrosis factor-alpha (TNF-alpha) in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages in a concentration-dependent manner. Incubation of LPS-stimulated macrophages with RES-UA NPs reduced the generation of reactive oxygen species (ROS) in a concentration-dependent manner. These results suggest that pH-responsive RES-UA NPs can be used to decrease ROS generation and inflammation.
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Collections - 4. Research institute > Nano-based Disease Control Institute > 1. Journal Articles
- 2. Clinical Science > Department of Orthopedic Surgery > 1. Journal Articles
- 2. Clinical Science > Department of Physical Medicine and Rehabilitation > 1. Journal Articles
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