Targeted Mutagenesis in Mice Using a Base Editor
- Authors
- Jeong, Tae Yeong; Lim, Soo-Yeon; Seong, Je Kyung; Kim, Kyoungmi
- Issue Date
- Jan-2023
- Publisher
- Humana Press Inc.
- Keywords
- Adenine base editor; CRISPR system; Cytosine base editor; Mouse model generation; Nucleotide substitution; Targeted mutagenesis
- Citation
- Methods in molecular biology (Clifton, N.J.), v.2606, pp 99 - 119
- Pages
- 21
- Indexed
- SCOPUS
- Journal Title
- Methods in molecular biology (Clifton, N.J.)
- Volume
- 2606
- Start Page
- 99
- End Page
- 119
- URI
- https://scholarworks.korea.ac.kr/kumedicine/handle/2021.sw.kumedicine/62832
- DOI
- 10.1007/978-1-0716-2879-9_9
- ISSN
- 1064-3745
1940-6029
- Abstract
- Base editors, such as cytosine and adenine base editors, are composed of nickase Cas9 (nCas9) and deaminase and serve as Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-based enzymatic tools for specific nucleotide substitutions. They are mainly the most effective genome editing tools for introducing point mutations, such as C-to-T and A-to-G conversions. The enhanced base editor, a C-to-G base editor (CGBE), can perform other nucleotide substitutions, such as C-to-G conversions. Here, we introduce a method for generating mouse models with point mutations using a base editing system. © 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
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- Appears in
Collections - 1. Basic Science > Department of Physiology > 1. Journal Articles
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