Enhanced behavioral assays in adult Zebrafish using an LED-based experimental tool

Abstract

Animals, which serve as models in human behavior and neuroscience research, offer invaluable insights into the intricate systems that underpin our actions and cognitive processes. Among the diverse array of organisms utilized in such studies, zebrafish are particularly promising subjects for behavioral experimentation. Although various behavioral assays have been developed to analyze neurological abnormalities in adult zebrafish, including anxiety, fear responses, cognitive impairment, and social interaction deficits, these assays are limited by numerous factors, including the spatial constraints of traditional cage setups, variability in experimental techniques, and the impact of animal handling on behavioral outcomes. To address these limitations, we developed a screen-based behavioral tool featuring LED displays positioned on either side of the experimental enclosure. Subsequently, we conducted a series of behavioral analyses, including assessments of learning, social interaction, fear, and preference in adult zebrafish, using this cutting-edge behavioral apparatus. This new approach effectively alleviates spatial constraints by projecting stimuli onto a screen and standardizing the experimental conditions across multiple researchers. Moreover, the screen-based setup minimized experimenter interference within the testing environment, ensuring improved consistency and objectivity in data collection. By overcoming traditional constraints and simplifying experimental procedures, this innovative approach marks a new era in behavioral research, enabling deeper insight into the complex mechanisms of behavior and cognition. © 2025 The Authors