상피성 난소암에서 프로테움 기법을 이용한 단백발현 분석

초록

OBJECTIVE The purpose of the present study is to identify the expression profiles of proteins in human epithelial ovarian cancers, as compared with those seen in normal ovarian tissues, via proteomic analysis. METHODS Three epithelial ovarian cancer tissues and three normal ovarian tissues were intraoperatively obtained. We performed two- dimensional electrophoresis in order to separate tissue proteins by molecular weight, and then compared protein expression patterns. 21 up-regulated spots were identified by MALDI-TOF in epithelial ovarian cancers. Then, the expression of some of the up-regulated proteins was evaluated at the mRNA level via RT-PCR, in both the epithelial ovarian cancer tissues and the normal ovarian tissues. RESULTS Proteomic analysis revealed about 200 up-regulated spots in epithelial ovarian cancer tissues, of which 21 were selected and identified by MALDI-TOF. 16 proteins were matched to MLC3nm, MACMARCKS, MYL2, S100A14, MIA, VHL, GUCA1B, RABL2A, BRMS1, IFI30, VILIP1, MAPRE1, NME5, DIO2, KLK 2, and CPA2. The up-regulation of these proteins was also evaluated at the mRNA level via RT-PCR, which revealed that MACMARCKS, S100A14, GUCA1B, RABL2A, VILIP1, MAPRE1, NME5, DIO2, and KLK2 were distinctly up-regulated in the cancer tissues. Five protein spots could not be matched, even with RT-PCR. CONCLUSION This proteomic analysis may constitute a powerful tool for the identification and characterization of many promising candidate proteins related to epithelial ovarian cancers.

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