이차구개형성과정에서 조직내 Fibronectin의 분포와 구개열유발인자가 이에 미치는 영향

초록

Distribution of immunofluorescence to fibronectin-specific antibody during normal development of secondary palate and in the process of the retinoid (isotretinoin) and steroid (triamcinolone acetonide) - induced cleft palate in the DDY mouse was studied to approach the role of fibronectin in palatogenesis. Fibronectin was present with the begining of formation of the palatal processes from maxilla and distributed throughout the mesenchyme, in the basement membrane and epithelia except medial edge portion, whereas it was almost not demonstrated. in the maxillary mesenchyme at this stage of development. Immunostaining intensity to fibronectin antibody was markedly increased in the whole area of the shelf just prior to elevation but the immunofluorescence was concentrated in the medial portion of the shelf with its elevation and localized in the medial edge epithelia and that of nasal septum during epithelial fusion and disappeared with the completion of parenchymal fusion. Isotretinoin and triamcinolone inhibited this increment of the immunoreactivity during the decisive stage of morphogenetic movement with consequent delay in palatogentic process, at least, shelf elevation. The results suggest that fibronectin involves in initiation of palatal shelf and plays a key role in palatogenetic processes that of shelf elevation and epithelial fusion.