흰쥐 골격근에서 허혈양상화에 의한 재관류손상의 방어기전에 관한 연구: Heat shock protein 70 및 c-fos mRNA 발현과 관련하여

초록

Recently due to an increase on the researches of the reperfusion injury of the skeletal muscles have proposed many methods on possibly reducing cellular injury. Of these, preconditioning have shown to be an effective method in reducing the reperfusion injury. There have been many hypotheses on the mechanism of preconditioning on reducing the reperfusion injury, but it still remains to be a focus of discussion. In this study, authors used c-fos mRNA known to be expressed under certain stimuli to promote the expression of a specific gene, and heat shock protein 70(hsp70) mRNA whose expression is increased under heat, trauma and ischemia to protect the cell from damage. Through the comparative observation in the expression pattern of c-fos and hsp mRNA in soleus(red) and gastrocnemius(white) muscles of rat in ischemia and reperfusion after preconditioning(5mins of ischemia and 5mins of reperfusion repeated 3 times), the authors have tried to find out the mechanism of preconditioning in reducing the reperfusion injury by in situ hybridization method. The expression of hsp70 mRNA peaked at 8hr ischemia in ischemia only (1) group, 2hr reperfusion in 2hr ischemia-reperfusion(2hrI-R) group, 1hr reperfusion in 4hr ischemia-reperfusion(4hrl-R) group. In preconditioning ischemia-reperfusion(PI-R) group, there was no temporal differences in relation to I-R group, however, there was an evident reduction in the intensity of mRNA expression. The expression in gastrocnemius muscle preceded that in soleus muscle. The c-fos mRNA was observed at 12hr ischemia in 1 group, no expression in 2hrl-R group, 1, 2 and 4hr reperfusion in 4hrl-R group. In PI-R group, the expression was observed at 1 and 2hr reperfusion in P2hrl-R group, and in all stages of P4hrl-R group. The mRNA expression was localized only to soleus muscle. From these results, it can be said that preconditioning depressed the expression of hsp70 mRNA and increased the expression of c-fos mRNA.

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