발생중인 구개조직에서 Collagen 및 HSPG의 분포와 구개열유발인자가 이에 미치는 영향에 관한 관찰

초록

Fluorescence immunohistocheulstry using collagen type Ⅲ, Ⅳ and heparan sulfate proteoglycan(HSPG)-specific antibodies was performed in the developing secondary palate of the DDY mouse and during the process of teratogen-induced cleft palate. Immunostaining with antibody to type Ⅲ collagen was present and localized in the basement membrane from the begining of formation of palatal shelf but it appeared in mesenchyme beneath the basement membrane during the mid-maturation period of the shelf and intensity of staining in mesenchyme was decreased prior to shelf elevation and disappeared both from basement membrane and mesenchyme with the shelf elevation: while triamcinolone and isotretinoin accelerated its decrement from both area. Type Ⅳ collagen and HSPG were demonstrated and localized in the basement membrane from the begining of shelf formation until its maturation and the fluorescence to type Ⅲ, Ⅳ and HSPG were not observed in epithelia during all stages of development. The results suggest that type Ⅲ and Ⅳ collagen involves in initiation and early maturation of palatal shelf but not in late maturation, elevation and fusion of the shelf.