급성 허혈성 신손상 백서에서 α-melanocyte stimulating hormone이 intercellular adhesion molecule-1의 발현및 신기능에 미치는 영향에 관한 연구

초록

Background : Acute renal failure is a reversible process in majority of cases but mechanisms of renal injury or recovery are poorly understood. Recently neutrophil infiltration is reported to potentiate inflammatory and cytotoxic cascade in ischemic renal injury and α- melanocyte stimulating hormone has been reported to have a potent anti- inflammatory properties in a variety of animal models. We examined the beneficial effects of α- MSH in acute ischemic renal injury in rats and tried to clarify its action mechanism. Methods : After unilateral nephrectomy, renal artery of contralateral kidney was clamped for 40 minutes and reperfused in female Sprague- Dawley rats. α- MSH (50μg) and vehicle was injected intraperitoneally immediately after and 6, 24 hours after reperfusion. Biochemical, histological data, ICAM- 1 mRNA, protein expressions and polymorphonuclear cell infiltration were examined. Results : α- MSH significantly attenuated the renal injury, measured by plasma BUN and creatinine level and also the degree of severity of histological injury (BUN 125.2±14.6 mg/dL : 46± 19.6 mg/dL (p=0.004), creatinine 3.65±0.81 mg/dL : 1.47±0.5 mg/dL (p=0.005) at 24 hours after reperfusion, BUN 88±12.5 mg/dL : 25.5±15.8mg/dl (p=0.002), creatinine 2.76±0.5 mg/dL : 0.93±0.2 mg/dL (p=0.002) at 72 hours after reperfusion and 5.4±1.94/ 2.6±0.77 (p=0.006) at 24 hours after reperfusion in histilogical grading system). In the α- MSH treated groups, ICAM- 1 mRNA expression decreased significantly compared to the vehicle treated ischemic group in 72 hours after reperfusion (0.49±0.01/0.31±0.2, p< 0.008). ICAM- 1 protein expression als o decreased in α - MSH treated group, but it was not statistically significant. Polymorphonuclear cell infiltrations howed a significant decrease in the α- MSH treated group at 24 hours after reperfusion (5.05± 1.8/1.59±0.4, p=0.009). Conclusion : α- MSH attenuates ischemic renal injury by inhibiting the expression of ICAM- 1 and subsequent polymorphonuclear cell infiltration. These results provide a rationale of α- MSH as a potential therapeutic drug in acute renal failure.

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