1-2 diglyceride를 기질로 한 Enzymatic rate법과 1,2-O-dilaurylrac- glycero-3-glutaric acid-(6-methylresorufin) ester를 기질로 한 Enzymatic Colorimetric 법에 의한 Lipase 측정 비교

초록

Lipases are glycoproteins with a molecular weight of 47000 daltons and are defined as triglyceride hydrolases. They catalyze the cleavage of diglycerides with the subsequent formation of monoglycerides and fatty acids. We evaluated serum lipase CX-7 Delta(1-2 diglyceride substrate) and Integra 700(1, 2-O-dilauryl-rac-glycero-3-glutaric acid-(6-methylresorufin) ester substrate). Within-run CVs of two level serums were 4.1% and 3.0%. The between-run CVs were 3.5%, 3.9%, respectively. The comparison between Integra700(y) and CX-7(x) resulted in a linear regression analysis described by the equation: y=3.002x+36.26 with a correlation coefficient r=0.9884.

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