백서의 두개골 결손부에서 제대혈 유래 간엽줄기세포의 골재생에 관한 연구

초록

It has been suggested that umbilical cord blood (UCB) contains multipotent stem cells. Some authors suggest that within UCB are cells with potential for differentiation down mesenchymal lineages and UCB is one of the readily available sources of stem cells that may be used for bone regeneration. Isolation and characterization of these cells has been accomplished in some centers. Differentiation of these cells down multiple lineages has been documented. The use of UCB derived stem cells was investigated in this animal studies. Polycaprolactone‐20% tricalcium phosphate (PCL‐TCP) polymers allow for cellular adherence and differentiation and are adequate scaffolds for bone regeneration in tissue engineering strategies. The purpose of this study was to evaluate the potential of MSCs from UCB seeded onto PCL‐TCP composite scaffolds for bone tissue engineering. MSCs were isolated from UCB. MSCs from UCB seeded onto PCL‐TCP scaffolds were then implanted in calvarial bone defect of the athymic mouse. As a control, in each mouse was treated with scaffold only. At six and twelve weeks post‐implantation, histological examinations were performed. MSCs from UCB with PCL‐TCP scaffolds did not exhibited bone regeneration in calvarial bone defects in athymic mouse. Histological examination did not reveal the presence of regenerated bone of the defect. MSCs from UCB should be considered further applications in tissue‐engineered bone regeneration.

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