겔 조성의 차이에 의해 유발되는 Apo B VNTR 유전좌위의 전기영동상의 변화

초록

It is always key point for typing polymarker that is determined by amplified fragment length polymorphism(AMP-FLP) with correct and precise detection method. Apo B VNTR locus is one of the widely used VNTR locus because of its high heterozygosity and large size(32 bp) of repeat unit. Its genotype can be determined by either non-denaturing polyacrylamide gel electrophoresis(ND-PAGE), denaturing polyacrylamide gel electrophoresis(denaturing PAGE). or agarose gel electrophoresis. But as it can be seen in this study, genotypes disregarding the factor of gel electrophoresis can cause non-equivalent result. Homozygous genotypes in agarose, denaturing PAGE system was turn out to be heterozygous genotype in the ND-PAGE. Sequence analysis showed 4 bp insertion/deletion at one site and substitution at the other site. It can be said that the different mobility was caused by the internal sequence differences between the two alleles. This phenomenon can be a serious problem for cooperative construction of databases. Thus, not only nomenclature but also the gel-electrophoresis system must be considered as a one of the factors to be standardized.