실험적으로 유발한 가토의 급성 상악동염에서 sialoglycoconjugates의 표현양상

초록

The present study was carried out in order to observe the changes in the expression of the sialoglycoconjugates of the rabbit sinus mucosa in the acute maxillary sinusitis model utilizing a biotin labeled lectins for light microscopy and lectin-gold complexes for eletron microscopy with three different lectins; Maackia amurensis (MAA), Wheat germ agglutinin (WGA) and Sambucus nigra (SNA). A comparison of the affinity of these lectins demonstrated the different distribution of sialoglycoconjugates in the ciliary layer and goblet cells. The normal sinus mucosa stained with three types of lectins showed that the sialoglycoconjugates were mainly distributed in the ciliary layer and goblet cells, and that their ultrastructural localization was restricted to the surface of the cilia, microvilli and secretory granules. Moreover, the main sugar residues of the sialoglycoconjugates were found to be consisted of Neu5Ac( 2,3)Gal, GlcNAc and Neu5Ac. Influenza A virus infection decreased the staining intensity of the mucosa with MAA, but not with WGA. In sinus mucosa infected with S. pneumoniae 7 days after Influenza A virus inoculation, however, these findings reverted to the staining intensity shown in the normal sinus mucosa. In addition, there was a hypersecretory activity of the hyperplastic and hypertrophic goblet cells which showed the intense reactivity with MAA and WGA. All of these changes in the staining intensity were also confirmed by the electron microscopic lectin-gold complexes. These results suggest that Neu5Ac(a2,3)Gal sugar residues may be required as protecting factor or modulator for Influenza A virus and s. pnumoinae infectivity.