간세포와 담낭상피의 분비성분 분포에 관한 면역조직학적 연구

초록

Variable human mucosal epithelium can externally secrete certain secretory antibodies when exposed to microorganisms or antigens. This concept is known as secretory immune system or local immunity and plays an important role in the protection of the mocosal epithelium against antigenic exposure. Local immunity(or mucosal immunity) is regulated by a special form of immunoglebulin A(IgA) and J-Chain, produced in submucosal layer by plasma cells, and secretory component (SC) produced by mucosal epithelial cells. The secretory component(SC) has the functional role as receptor for IgA in facilitating diffusion of IgA into the lumen as complete secretory IgA. Detection of the secretory component in the cytoplasm of epithelial cells using immunohistological method suggests the function of the epithelial cell as mucosal immunity(or local immunity). This study aimed to find and compare the distribution of SC in hepatocytes, gallbladder and bile duct epithelial cells in normal and diseased conditions of the hepatobiliary system. Tissues were obtained by needle biopsy or surgical removal of the liver or gallbladder. 23 case5 of formalin-fixed and paraffin-embeded liver tissue(normal liver, 5 cases; active viral hepatitis, 5; Chronic active hepatitis, 5; hepato cellular carcinoma, 3 and cholangiecarcinoma. 3) and 18 cases of gallbladder (normal gallblader, 5; cholecystitis without stone, 6 and adenocarcinoma, 2) were selected and immunoperoxidase staining(Avidin-Biotin-Complex method) was performed using anti SC serum. The intensity of the stain and number of antigen containing cells were then observed. The results are as follows: 1. Normal liver tissue, biliary duct and gallbladder epithelium showed diffuse and strong SC positive reaction. 2. Cells of hepatocellular carcinoma, cholangiocarcinoma and adenocarcinoma of the gallbladder showed diffuse and strong SC positive reaction similar to normal hepatocyte and biliary epithelial cells. 3. SC positive cells were decreased in hepatitis in comparison with normal liver tissue and markedly decreased in chronic active ehepatis. 4. Gall bladder epithelial cells revealed different SC positive reaction in cholescystitis between without and with gall stone. All cases showed diffuse, strong SC positive reaction in cholecystitis without gallstone, and 4 out of 6 cases showed SC negative reaction in cholecystitis with gall stone. Above findings suggest that normal hepatocyte, biliary epithelium and epithelium of gall bladder and carcinoma arising from hepatobiliary system produce equally the secretory component(SC). Hepatocytes damaged in hepatitis may lose the cellular function of the production of secretory componet(SC) and it seems to be closely related to the SC negative reaction of the epithelial cells of gallbladder and formation of gallstone.

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