Mechanism of action of 9-(1,3-dihydroxy-2-propoxymethyl) guanine for its anti-herpes activity: Inhibition of viral DNA and protein synthesis in cells infected with herpes simplex virus

초록

In the present study we examined the effect of antiviral agent 9-(1,3-dihydroxy-2-propoxymethyl) guanine(DHPG) on herpes simplex virus type 1(HSV-1) DNA and protein synthesis during virus replication to understand its mechanism of antiherpetic action DHPG, in a concentration of 1 μM, markedly and selectively inhibited the synthesis of viral DNA in HSV-1 infected cells, but in higher concentration also suppressed the synthesis of cellular DNA. Treatment of virus infected cells with DHPG significantly increased thc synthesis of beta proteins in the later stage of viral infection. However, DHPG notably or completely diminished the level of gamma proteins, and did so in a dose dependent fashion. The increase in the expression of beta gene products was most likely a response to the inhibition of gamma proteins synthesis by DHPG. The reduction of the synthesis of gamma proteins by DHPG may due to the inhibition of progeny viral DNA synthesis. We conclude that DHPG primarily inhibits the synthesis of viral DNA. and by doing so exhibits its antiherpetic activity. The alteration in viral proteins synthesis in the presence of DHPG might result from the alteration in viral DNA synthesis

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