마우스 상피세포의 일차배양시 배양양상과 최적조건에 관한 연구

초록

This study was attempted to compare the characteristics of primary culture of mouse epithelial cells and to determine the effective seeding density for confluency on two substrates. The dispersed single epithelial cells were cultured on culture dish coated with type Ⅲ collagen and culture slide. The culture conditions were adjusted to 3 DMEM:1 Fl2 media with 10% FBS, 37℃ temperature. 5% CO₂ air and pH 7.2-7.4 but the seeding density was changed within 1×10⁴-7×10^5 cells / ㎠ substrate. The results were as follows: 1. The characteristics of cultured cells were very similar in the collagen coated dish-used group and the culture slide-used group. The proliferation, stratification, keratinization and desquamation processes were observed like those in vivo. 2. There was a slight tendency to promote attachment. proliferation and differentiation on the collagen coated dish as compared with the culture slide. But it was not statistically significant. 3. The effective seeding density was in the ragne of 4-5×10^5 cells/㎠ substrate in both groups. But the time for cofluency was shortened accordings to rasing the seeding density.