The viability enhancement of encapsulated cells in alginate beads using hydrodynamic removal of toxic oleic acid

초록

In this paper, we propose a microfluidic chip to improve cell viability after encapsulation in alginate bead used for biological assays such as stem cell differentiation and anti-cancer drug sensitivity. We added a flushing channel to exchange toxic oleic acid with mineral oil to improve cell viability. The flushing of oleic acid after the gelation of alginate dramatically increased the viability of P19 embryonic carcinoma cell up to 90%. The cell viability was proportional to the flow rate of squeezing mineral oil. © 2008 CBMS.