비호지킨씨 림프종에서 세포분열수, Ag-NORs 및 유세포 측정법을 이용한 세포증식능 평가에 관한 연구

초록

In non-Hodgkin's lymphomas, it has been well known that the prognosis of the disease depends on the clinical stage and therapeutic modality as the histologic grade. Histologic assessment of proliferating activity is important in grading and classification of the non-Hodgkin's lymphomas. To evaluate the relationship between conventional method(counting the mitoses), argyrophilic method(Ag-NORs) and attractive, but expensive and complicate method(flow cytometry), the forty cases of non-Hodgkin's lymphomas, which had been routinely processed and immunophenotypically classified, were studied. The numbers of the mitoses and Ag-NORs were counted in all 40 and in thirty-one cases among them DNA ploidy and cell cyclic study were done. The results were summarized as follows : 1. The mean numbers of mitoses and Ag-NORs were significantly different between low and intermediate grades, low and high grades, and intermediate and high grades of non-Hodgkin's lymphomas. However, there were no significant differences between each histologic grades in the results of cell cyclic study using flow cytometry. 2. Between T and B cell lymphomas, there were no significant differences of the mean numbers of mitoses and Ag-NORs as well as the results of cell cyclic study using flow cytometry. 3. There was a positive correlation between numbers of mitoses and Ag-NORs. However, no correlation was found between the proportion of the cells in each cell cycle and the mean numbers of mitoses as well as the mean of Ag-NORs numbers. 4. Among 31 non-Hodgkin's lymphomas in which flow cytometric analysis was done, 20 cases showed aneuploidy pattern of DNA. However, there was no difference in the incidence of aneuploidy between each histologic grades as well as functional classes.