BSA, Glucose및 Phosphate가 1세포기 생쥐수정란의 체외 배양시 2-cell block과 배 발달에 미치는 영향

초록

Objective : To assess the effect of BSA, glucose and phosphate addition to a chemically defined medium (M16) on the development of mouse one-cell embryos in vitro. Method : Harvested mouse (ICR) one-cell embryos were cultured for 48 hours using M16 solution with various concentrations (0-15%) of BSA. First, mouse 1-cell embryos were devided into two groups. One group of them was cultured under the same condition for further 72 hours and the other group was cultured in the media with fixed concentration (0.3 %) of BSA, and evaluated the effect of the media on the 2-cell block and further embryonal development. Secondly, the effects of removal of glucose and/or phosphate from the BSA conditioned culture media (M16) on the development of mouse embryo were also evaluated. Results : Higher concentrations (0.9-1.5 %) of BSA in the culture media during the first 48 hours of 1-cell mouse embryo culture overcome 2-cell block significantly, however, the continued exposure to higher concentrations after 48 hours do not influence further development. Removal of glucose and/or phosphate from the culture media during the early stage (first 48 hours) of culture showed significant overcoming of 2-cell block and increased the blastulation rate, but after the first 48 hours of culture, the absence or presence of glucose and/or phosphate do not influence the further embryonal development.Finally, the positive effects of the removal of glucose and/or phosphate are also augmented with the increased BSA concentration of the culture media. Conclusion : Increased BSA concentration and removal of glucose and/or phosphate during the early stage of culture may have favorable effect on 2-cell block and further development of mouse embryo in vitro.

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