Dietary Supplementation with Bacillus Superoxide Dismutase Protects against Contrast-Induced AKI in Mice

초록

Background: Superoxide dismutase (SOD) is a metalloenzyme that play an important role in antioxidant defense against oxidative stress. Oxidative stress was known as one of the main pathophysiology in contrast-induce acute kidney injury (CI-AKI). Based on recent data showing the important role of gut dysbiosis, using probiotics could have a potential to modify systemic oxidative stress. We investigated the renoprotective potential of orally administered enteric-coated SOD protein purified from Bacillus amyloliquefaciens (SOD-BA) in contrast-induced acute kidney injury (CI-AKI) in mice. Methods: CI-AKI model was induced with unilateral nephrectomy using 5 week-old C57BL/6 male mice. Giving oral gavage SOD-BA once a day, 48 hours before intravenous contrast injection (10mg/kg) 4 weeks after unilateral nephrectomy. Serum blood urea nitrogen (BUN), creatinine, and kidney tissue NGAL were determined. Results: SOD-BA did not have kidney toxicity in the vehicle group. The unilateral CI-AKI model showed minimal kidney function deterioration based on creatinine level. However, the serum BUN and NGAL were strikingly increased. SOD-BA oral administration group showed decreased BUN and kidney NGAL level. Conclusion: Prophylactic treatment of oral SOD-BA could serve as a potent antioxidant in the prevention and treatment of CI-AKI.