Antibacterial Effect of Antibiotic-Saturated Fibrin Sealant; In Vitro Study

초록

Background Many chronic wounds have inadequate blood supply around the wounds and systemic antibiotics are less efficient. Topical antiseptic can cause cytotoxicity and irritation or allergic dermatitis. Therefore, topical antibiotics that can sustain their effects in an extended period are often needed to replace topical antiseptics. The present study evaluates the effect of antibiotic-saturated fibrin sealant against Methicillin-Resistant Staphylococcus Aureus (MRSA) by adjusting the concentration of the antibiotic as well as the duration exposure. Methods Teicoplanin antibiotic was impregnated in the fibrin disc at concentrations of 50 µg/mL, 100 µg/mL, 200 µg/ mL, 400 µg/mL and control (0 µg/mL). The samples were incubated at 37ºC in CO2 incubator for 7, 14, 21, and 28 days. After the incubation period, the antibiotic-saturated fibrin discs were transferred to Mueller-Hinton agar surface scrubs with MRSA. The antimicrobial susceptibility testing was carried out according to Clinical and Laboratory Standards Institution document and using filter papers. Results The antibiotic-saturated fibrin disc showed a greatest antibacterial activity during the first week. However, the 50µg/mL groups fell below suitability within 14 days. Other groups maintained their effect for 28 days, and these 3 groups showed suitable effects. Conclusion Antibiotic-loaded fibrin sealants may act as vehicles to deliver antibiotics in a local wound infection. Optimal concentration of antibiotics is 100 µg/mL.

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