Differential Susceptibility of Myelin Binding Protein and Myelin Oligodendrocyte Glycoprotein Antibody between Optic Nerve and Central Nerve In Animal Model.

초록

Purpose : To conduct a comparative analysis of the activities of Myelin Binding Protein (MBP) and Myelin Oligodendrocyte Glycoprotein (MOG) to investigate the anatomical and physiological characteristics of myelin sheaths in both optic nerve and brain within a rat model. Methods : Tissues, including the optic nerve and a random brain tissue sample, were extracted from eight Sprague-Dawley rats. The activities of MBP and MOG were analyzed using immunochemical staining and western blot analysis. Immunohistochemistry was used to qualitatively assess MBP and MOG expression levels. Immunochemical staining intensity was quantified using the ImageJ software to provide an accurate and quantifiable representation of MBP and MOG expression levels. Results : Immunological analysis showed that the intensities of MBP and MOG staining were more prominent in the optic nerve than in the brain tissue. In addition to qualitative analysis, we quantified the stained antibody intensities (MBP and MOG) using Image J software. The staining intensities of MBP and MOG in the optic nerve were significantly higher than those in the brain. (52.85 ± 7.75% in optic nerve and 14.17 ± 5.04% in brain) (52.65 ± 7.96% in optic nerve and 14.17 ± 5.04% in brain) (p < 0.01). Protein levels of MBP and MOG were measured by western blot analysis, and significant increases in both MBP (p < 0.01) and MOG (p = 0.02) protein levels were observed in the optic nerve relative to the brain tissue. Conclusions : MBP and MOG proteins are selectively expressed in the optic nerve compared to other CNS tissues in animal models. This is believed to be the anatomical and physiological basis of demyelinating lesions that prominently affect the optic nerve.