A myristoylated pseudosubstrate peptide of PKC-ζ induces degranulation in HMC-1 cells independently of PKC-ζ activity

Lim S.; Choi J.W.; Kim H.S.; Kim Y.-H.; Yea K.; Heo K.; Kim J.H.; Kim S.-H.; Song M.; Kim J.I.; Ryu S.H.; Suh P.-G.

doi:10.1016/j.lfs.2008.01.005

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A myristoylated pseudosubstrate peptide of PKC-ζ induces degranulation in HMC-1 cells independently of PKC-ζ activity

Lim S.;
Choi J.W.;
Kim H.S.;
Kim Y.-H.;
Yea K.;
외 7명

Citations

SCOPUS

19

초록

Mast cells play a central role in allergic disease and host defense against several pathogens through the release of various bioactive compounds via degranulation. In this study, we found that a myristoylated pseudosubstrate of PKC-ζ (ζ-PS; myristoyl-SIYRRGARRWRKL, a PKC-ζ inhibitor) regulates mast cell degranulation. ζ-PS increased [Ca+2]i level at nanomolar concentrations in a PKC-ζ activity-independent manner in HMC-1 cells. Moreover, ζ-PS-induced [Ca+2]i generation was completely abrogated by phospholipase C (PLC), IP3 receptor or Gαi/o inhibitor and ζ-PS potently induced degranulation in HMC-1 cells which was significantly inhibited by pretreating PLC inhibitors or a calcium chelator. Therefore, our results suggest that ζ-PS can induce degranulation in HMC-1 cells by triggering the calcium signal via a PKC-ζ-independent but Gαi/o, PLC and IP3-dependent pathways. © 2008 Elsevier Inc. All rights reserved.

키워드

ζ-PS; Calcium; Degranulation; IP3; Mast cells; Phospholipase C; chelating agent; peptide; phospholipase C; phospholipase C inhibitor; protein kinase C zeta; animal cell; article; calcium cell level; calcium mobilization; calcium signaling; confocal laser microscopy; controlled study; enzyme activity; human; human cell; immune response; mast cell; mast cell degranulation; mouse; myristylation; nonhuman; beta-N-Acetylhexosaminidases; Calcium; Calcium Signaling; Cell Degranulation; Cell Line; Enzyme Inhibitors; GTP-Binding Protein alpha Subunits, Gi-Go; Humans; Inositol 1,4,5-Trisphosphate Receptors; Ligands; Mast Cells; Microscopy, Confocal; Oligopeptides; Peptide Library; Protein Kinase C; Receptors, G-Protein-Coupled; Substrate Specificity; Type C Phospholipases

제목: A myristoylated pseudosubstrate peptide of PKC-ζ induces degranulation in HMC-1 cells independently of PKC-ζ activity

저자: Lim S.; Choi J.W.; Kim H.S.; Kim Y.-H.; Yea K.; Heo K.; Kim J.H.; Kim S.-H.; Song M.; Kim J.I.; Ryu S.H.; Suh P.-G.

DOI: 10.1016/j.lfs.2008.01.005

발행일: 2008

유형: Article

저널명: Life Sciences

권: 82

호: 13-14

페이지: 733 ~ 740

언어: ENG

출판사: Elsevier BV

발행국가: 영국

분량: 8 페이지

ISSN: E 1879-0631
P 0024-3205