Effect of proton pump inhibitor on the bone turnover in the ovariectomized ICR mice

초록

Introduction Taking PPIs for more than a year was associated with increased fracture risk by 44% while long-term, high-dose users had 2.6 times greater risk than nonusers, reported. Aims and Methods The aims of this study were to evaluate the effect of PPI on the bone turnover in the ovariectomized ICR mice. The 8-week-old ovariectomized female ICR mice were randomized into either PPI group or control group. PPI groups (n =10) and control groups (n =10) were injected with pantoprazole (40 mg/kg/day, i,p) or normal saline (i,p) for 4 weeks respectively. Bone mineral density (BMD) at tibia, relative tibia weight and serum chemistries (ALP, Ca, Phosphorus) were measured. Serum levels of osteocalcin and CTX-1 were measured by ELIZA. The cytokines such as IL-1b, IL-6, and TNF-α were determined by RT-PCR. Results There were no differences in BMD, tibia relative weight and serum chemistries between two groups (p >0.05). Serum levels of osteocalcin in pantoprazole groups were significantly lower than that of control groups (p =0.023), but no difference found at the serum levels of CTX-1(p >0.05). The expression of IL-1b in spleen was suppressed significantly in pantoprazole group, but there were no significant differences at the expressions of TNF-α and IL-6 between two groups. Conclusion The pantoprazole did not affect the bone mineral density, serum chemistry and osteocalcinogenesis in the ovariectomized ICR mice. However, the pantoprazole might have a possibility to effect on the bone turnover by the suppression of osteoblastogenesis.