Effects of vibration on the proteome expression of anterior cruciate ligament cells

초록

Recent reports have suggested that vibration has beneficial effects onknee healing response; however, the biomechanism of these beneficialeffects still need to be determined on the anterior cruciate ligament(ACL) cell level. In this study, we applied a 20 Hz vibration to ACLcells, which produced a 20% increase (p < 0.001) in cell activity and17% incr ease (p < 0.001) in intracellular sulfated glycosaminoglycan(GAG) levels. In the 20 Hz vibration-stimulated ACL cells group, eightup-regulated (100~300%) protein spots were identified compared tothe control group by proteomics analysis. Among these proteins, Ann-exin A2 and Prolyl 4 hydroxylase (PH4B) were shown to have a 71%and 16% higher expression, respectively, in the 20 Hz vibration-stimu-lated ACL cells group by western blotting (p < 0.001). These resultsindicate that vibration produces a positive cellular environment, andAnnexin A2 and P4HB are expected to help ligament repair and ACLcell proliferation by controlling cell membrane and extra cellularmatrix formation.