식물응집소와 항리소자임항체의 조직구에 대한 면역조직화학적 연구

초록

Histiocytes have the characteristics of phagocrtosis, secretory activity and antigenic processing in immunologic and nonimmunologic reactions. Now the classification and diagnosis of histiocytic proliferative disorders are so complex and difficult that there have been many efforts to discover the histiocytic markers such as lysozyme, α₁-antitrypsin, and α₁- antichymotrypsin. Recently the possibility of lectins as a histiocytic marker, especially Peanut agglutinin (PNA) and Ricinus communis agglutinin I (RCA-I), have been reported. The author applied several lectins to benign and malignant histiocytes by avidin-biotin-complex (ABC) method and compared it to antilysozymal antiserum. Included here are 6 cases of reactive hyperplasia of lymph node, 11 cases of malignant histiocytosis and 2 cases of eosinophilic granuloma. The results are as followings; 1. PNA revealed positive reaction to all benign histiocytes in reactive hyperplasia and negative reaction to 9 of 11 malignant histiocytes in malignant histiocytosis. 2. RCA-I revealed positive reaction to benign histiocytes and negative reaction to 5 of 6 cases of malignant histiocytosis. 3. WGA and Con A revealed positive reaction to all benign and malignant histiocytes. 4. Eosinophilic granuloma revealed similar reaction to benign histiocytes to each lectin. 5. Antilysozomal antibody revealed positive reaction to 4 of 6 cases of reactive hyperplasa, 2 of 8 cases of malignant histiocytosis and two cases of eosinophilic granuloma.

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