타액선 암종세포의 식물 응집소 수용체 분포에 관한 면역조직화학적 연구

초록

Malignant tumors of the salivary glands are variable in their histologic pictures, and their histochemical changes in membrane carbohydrate composition have not been fully understood. The author selected formalin fixed, paraffin embedded normal tissue and malignant tumors of salivary gland, and performed immunohistochemical study on the selected tissue materials by avidin biotin peroxidase complex method using lectins such as Concanavalin A (Con A), Soybean agglutinin (SBA), Wheat germ agglutinin (WGA), Dolichos biflorus agglutinin (DBA), Ulex europaeus agglutinin I (UEA I), Peanut agglutinin (PNA), Ricinus communis agglutinin I (RCA I), and Pinellia ternata agglutinin (PTA). The results are summarized as follows: 1. Lectins showing different reaction pattern between normal and malignant tumor cells of the salivary glands were DBA, UEA-1, SBA and PNA. 2. DBA and SBA showed positive reaction with normal duct epithelium, but they did'nt react with malignant turner cells. 3. UEA-1 revealed positive reaction with normal duct epithelium and cancer cells of the mucopeidermoid carcinoma. But it did'nt react with adenoid cystic carcinoma cell and acinic cell carcinoma cell. 4. PNA showed positive reaction with mucoepidermoid carcinoma cell and negative reaction with nor-mal duct epithelium, adenoid cystic carcinoma and acinic cell carcinoma cell. Above findings suggest loss of N-acetyl-galactosaminyl moiety in malignant tumors of salivary gland, loss of L-fucosyl in adeniod cystic cacinoma and acinic cell carcinoma, which are present on normal duct epithelium, and appearance ofβ-D-galactosyl moiety in mucoepidermoid carcinoma which is absent on normal duct epithelium.

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