Detection of genetic alterations in Korean ovarian carcinomas by degenerate oligonucleotide primed polymerase chain reaction-comparative genomic hybridization

초록

Chromosomal aberrations in 22 Koreans with ovarian carcinomas were investigated by degenerate oligonucleotide primed-polymerase chain reaction comparative genomic hybridization. The common sites of copy number increases were 20q (90%), 17q23similar toqter (86%), 8q22similar toqter (68%), 3q25similar toqter (59%), 6p21 (59%), 11q13 (54%), 16p (40%), 2q31similar toqter (36%), 7q (36%), 14q31 (36%), 15q24similar toqter (36%), and 1q32similar toqter (31%). DNA amplification was identified in 18 carcinomas (82%). The frequent sites of amplification were 20q13.2similar toqter, 8q24.1, 17q23similar toqter, 3q25similar toqter, and 6p21. The most frequent sites of copy number decreases were 4q21similar toq31 (54%), 5q13similar toq21 (50%), and 13q14similar toq21 (45%). The recurrent gains and losses of chromosomal regions identified in this study provide candidate regions that may contain oncogenes or tumor suppressor genes, respectively. (C) 2003 Elsevier Inc. All rights reserved.

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