IMPACT OF DIMENSIONALITY AND EXTRACELLULAR MATRIX ON MESENCHYMAL STEM CELL PHENOTYPE

초록

Background: Umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) possess self-renewal, multi-differentiation, and tissue regenerative capabilities, making them a promising candidate for various disease treatments. However, the role of the extracellular matrix (ECM) in the microenvironment remains poorly understood. In this study, we aimed to assess the impact of the ECM on phenotypic changes based on cell dimensionality and ECM types. Methods: To analyze phenotypic differences, UCB-MSCs were cultured in low attachment microwells for 24 hours to form spheroids. These spheroids were then embedded in either no ECM, type I collagen, or Matrigel, and cultured for 48 hours with or without IFN-gamma. The harvested cells were compared to those of the monolayer. Results: Depending on cell dimensionality and types of ECM, UCB-MSCs exhibited significant variations in the expression of PDL1, HLA-DR, and IDO, indicating distinct phenotypes. Conclusions: The phenotype of UCB-MSCs is believed to be strongly influenced by filament rearrangement and antigenicity depending on cellular dimensionality and ECM type. Disclosure: No conflict of interest in this abstract.