Guanethidine이 백서적출심장의 catecholamine 유리에 미치는 효과

초록

The isolated heart of matured rat in the perfusion experiments respond to continuous infusion of guanethidine with an increase in heart rate. This agent appear to increase heart rate by releasing endogenous norepinephrine, since depletion of cardiac norepinephrine store abolishes the action. In the present studies, to inverstigate the role of guanethidine on the release of catecholamine, the author observed the influence of guanethidine on the variations of catecholamine contents in the myocardium and perfusate from the perfusion experiment of isolated rat heart. Perfusions were carried out with varying concentration(1㎎, 5㎎ and 10㎎ per 1L of Tyrode solution) of guanethidine for 1 hour, 2 hours and 3 hours, according to Langendorff method. The results were as follows; 1. The norepinephrine content of isolated myocardium was decreased by the perfusion of guanethidine. 2. The norepinephrine content in the perfusate from isolated myocardium was increased by the perfusion of guanethidine. 3. Total norepinephrine content in both of isolated myocardium and the perfusate from isolated myocardium was increased progressively by the perfusion of increasing concentration of guanethidine in Tyrode solution and the prolongation of perfusion.