韓國人에서 重合酵素反應으로 分析한 DXS52 遺傳座位의 遺傳的 多樣性

초록

The DXS52 locus was studied in a Korean population sample(n=142) for its allele and genotype frequencies and applicability to identity and paternity testing, using polymerase chain reaction with electrophoresis of PCR products and subsequent detection of allelic fragments by ethidium bromide staining. In Korean population, 15 alleles with the frequency range of 0.319-0.005 are detected in the DXS52 locus, and the highest allele frequency among them is found to be allele 1(0.319). The correlation coefficient between gene frequencies detected in female in mali is 0.9334, indicating that the gene frequency in males follows that of females in Korean population. This locus studied meets Hardy-Weinberg expectation : there is good agreement between the observed and expected values under Hardy-Weinberg equilibrium. The genotypes 1-12(0.118) are mostly frequent found among the 29 genotypes detected in Korean female, but the 91 genotypes are not found. The observed heterozygosity for this locus is 0.809, while the unbiased expected estimate of heterozygosity is 0.827 calculated by the expectation of Hardy-Weinberg equilibrium(p>0.05). The power of discrimination(PD) and power of exclusion(PEX) calculated for this locus is 0.9770(97.70%) and is 0.7068(70.67%), respectively. Thus, the allelic and genotypic data estimated for DXS52 locus can be used as the database of PCR-based DNA profile to human identity testing.