Application of differential expression of genetic profiles in brain tumors with variable [F-18]-fluorodeoxyglucose uptak

초록

F-18-fluorodeoxyglucose (FDG) uptake on positron emission tomography (PET) scan has been found to reflect tumor aggressiveness and prognosis in various types of cancer. In this study, the gene expression profiles of glial tumors were evaluated to determine whether glial tumors with high F-18-FDG uptake have more aggressive biological potential than with low uptake. Surgical specimens were obtained from the 12 patients with glial tumors (4 males and 8 females, age range 42-68 years). The tumor samples were divided into two groups based on the F-18-FDG uptake PET scan findings: high F-18-FDG uptake (n =4) and low F-18-FDG uptake (n=8). The pathological tumor grade was closely correlated with the F-18-FDG uptake pattern: Glial tumors with high 18F-FDG uptake were pathologically Edmondson-Steiner grade III, while those with low uptake were grade II. The total RNA was extracted from the frozen tissues of all glial tumors (n=12), and adjacent non-cancerous tissue (n=3). The gene expression profiles were evaluated using cDNA microarray. The glial tumors with high 18F-FDG uptake showed increase expression of 15 genes compared to those with low uptake (P< 0.005). Nine genes were down-regulated. Gene expression is closely related to cell survival, cell-to-cell adhesion or cell spreading; therefore, glial tumors with high F-18-FDG uptake appear to have more aggressive biological properties than those with low uptake.

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