CDK4 down-regulation induced by paclitaxel is associated with G(1) arrest in gastric cancer cells

초록

Paclitaxel induces a cell cycle block at G(2)-M phase by preventing the depolymerization of microtubules and induces p53-independent apoptosis in many cancer cells, We observed that gastric cancer cells treated with paclitaxel have shown a cyclin-dependent kinase (CDK)4 do down-regulation, This paclitaxel-induced CDK4 down-regulation resulted in a cell cycle arrest at G(1)-S phase. To confirm this observation,,ve prepared stable transfectants that overexpressed CDK4 and analyzed the cell cycle progression. Ectopic expression of CDK4 in SNU cells resulted in a release of paclitaxel-induced G(1) arrest. The release of G(1) arrest by enforced expression of CDK4 seems to make the cells more sensitive to paclitaxel-induced apoptosis. From this finding, we could then suggest that paclitaxel treatment induces both G(1)-S and G(2)-M blocks in the cell cycle progression of gastric cancer cells.

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