Subtype classification of gastric spindle cell tumors in whole slide images

초록

Aims Accurate cancer subtype classification is critical due to variations in tumor progression and prognosis. Traditionally, pathologists classified subtypes manually by examining pathological slides under the microscope. To address increasing workloads, this study aimed to classify gastric spindle cell tumor (GSCT) subtypes—gastrointestinal stromal tumors (GIST), leiomyomas, and schwannomas—using a convoluted neural network applied to whole-slide images (WSIs). To date, no automated pipeline has been proposed for classifying GSCTs into GIST, leiomyoma, and schwannoma from hematoxylin and eosin (H&E) WSIs. The limited number of leiomyoma and schwannoma cases necessitates training on class-imbalanced datasets, which can cause performance degradation for minority classes and model overconfidence. Methods and results We applied CutMix operations to generate datasets for minority classes, constructing a balanced dataset that improved F1 scores for leiomyoma and schwannoma from 0.9187 to 0.9651 and 0.9258 to 0.9808 in internal validation, and from 0.6004 to 0.8795 and 0.4792 to 0.6181 in external validation, respectively. Additionally, considering model overconfidence, we employed Confident Instance Voting (CIV) on instances with confidence exceeding 0.9, unlike conventional hard voting, achieving accuracy from 0.9680 to 0.9826 and from 0.9138 to 0.9262 in internal and external validation. We propose a first fully automated pipeline for classifying GSCTs into three classes from hematoxylin and eosin (H&E) WSIs using the aforementioned approaches. Conclusions This pipeline demonstrates potential as a clinical tool for categorizing gastric spindle cell tumor subtypes. It offers improved diagnostic efficiency and reliability, particularly in addressing class imbalance in histopathological analysis. © 2025 The Authors.

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