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Neural cell fate analysis in zebrafish using olig2 BAC transgenics
- Shin J.;
- Park H.-C.;
- Topczewska J.M.;
- Madwsley D.J.;
- Appel B.
Citations
SCOPUS
241초록
We describe here simple methods for producing transgenic zebrafish reporter lines using BAG clones. The use of BAG clones facilitates creation of useful transgenics as the large amounts of genomic DNA they contain increase the likelihood that reporter gene expression will be properly regulated. Combined with recent advances in live embryo image analysis, this strategy has the potential to greatly advance the investigation of neural cell behavior during development.
키워드
Cerebellum; EGFP; Motor neuron; Olig2; Oligodendrocyte; Purkinje neuron; Spinal cord; Transgenic; Zebrafish; enhanced green fluorescent protein; genomic DNA; green fluorescent protein; unclassified drug; animal cell; animal experiment; article; bacterial artificial chromosome; brain development; cell clone; cell fate; controlled study; embryo development; embryology; gene; gene expression; gene expression regulation; genomics; nerve cell; nerve cell differentiation; nonhuman; oligodendrocyte gene 2; reporter gene; transgenics; zebra fish; Animals; Cloning, Molecular; Embryo, Nonmammalian; Gene Expression Regulation, Developmental; Genes, Reporter; Genetic Vectors; Green Fluorescent Proteins; Luminescent Proteins; Microinjections; Motor Neurons; Peripheral Nervous System; Purkinje Cells; Zebrafish; Animalia; Bacteria (microorganisms); Danio rerio; Vertebrata
- 제목
- Neural cell fate analysis in zebrafish using olig2 BAC transgenics
- 저자
- Shin J.; Park H.-C.; Topczewska J.M.; Madwsley D.J.; Appel B.
- 발행일
- 2003
- 유형
- Article
- 권
- 25
- 호
- 1-2
- 페이지
- 7 ~ 14
- 언어
- ENG
- 출판사
- Kluwer Academic Publishers
- 발행국가
- 네덜란드
- 분량
- 8 페이지
- ISSN
- P 1381-5741