간접형광항체법 면역효소측정법 및 고비중입자응집반응에 의한 신증후출혈열 환자 한탄바이러스 예방백신 접종자 및 실험동물 혈청에 대한 항체비교

초록

This study was intended to evaluate the availability of the high density composite particle agglutination(HDPA) test for serological diagnosis of hemorrhagic fever with renal syndrome (HFRS) compared to indirect immunofluorescent antibody(IFA) technique and enzyme-linked immunosorbent assay(ELISA). With sera from HFRS patients, individuals vaccinated with Hantaan virus and experimental animals infected or immunizied with Hantaviruses studies were carried out to define the correlation of HDPA test with IFA test, to compare the antibody titers measured by IFA ELISA and HDPA tests, and to evaluate the cross-reactivity and reaction patterns of HDP coated with Hantaan virus antigens. The results were summarized as follow: 1. High agreement (%agreement=98.3%) rate was observed between results of serological diagnosis of HFRS patients by HDPA and IFA tests. 2. Highly significant correlation (r =0.8826, p<0.0001) was observed between antibody titers measured by HDPA and IFA tests. 3. Antibody titers of serial bled sera from HFRS patients were in the following order: IgM-ELISA ≥ HDPA > IFA in febrile phase of diseases and HDPA ≥ IgM-ELISA≥IFA in convalescent phase of diseases. 4. Positive rates of sera from vaccinees by HDPA and IFA tests were 12/71(17%). 10/71(14%) on 4650days after Hantaan virus vaccination and 65/71(92%), 64/71(90%) on 10days after booster injection of vaccine. 5. With HDP coated with Hantaan virus antigens high crossreactivity was observed in sera from HFRS patients infected with Seoul virus, and low cross-reactivity infected with Puumala virus. 6. With HDP coated with Hantaan virus antigens strong positive agglutination patterns were observed in sera from striped field mice, poor agglutination patterns in sera from house and laboratory rats, moderate agglutination patterns in sera from ICR and Balb /cmice, guinea pigs and rabbits.