Cell density-dependent acetylation of Delta Np63 alpha is associated with p53-dependent cell cycle arrest

초록

Delta Np63 alpha is a p63 isoform that is predominantly expressed in the epidermal stem cells and in cancer. To find the regulatory pathways of Delta Np63 alpha, we assessed whether Delta Np63 alpha is acetylated and determined the functional implications of acetylation. First, the hinge region of p63 was shown to be acetylated by PCAF, similarly to other p53 family members. Second, acetylation synergistically induced cytoplasmic localization of Delta Np63 alpha. Finally, acetyl-Delta Np63 alpha was induced during high-density culture, suggesting that acetylation of Delta Np63 alpha may reinforce cell cycle arrest upon cell contact. Altogether, these findings suggest that acetylation of Delta Np63 alpha contributes to the epidermal homeostasis. Structured summary of protein interactions: PCAF acetylates Delta Np63 alpha by acetylation assay (View Interaction: 1, 2, 3) Delta Np63 alpha physically interacts with Delta Np63 alpha by anti tag coimmunoprecipitation (View interaction) Delta Np63 alpha physically interacts with p53 by anti bait coimmunoprecipitation (View interaction) (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

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