가토 두개부 골결손부 우골 유도 골이식재의 이식시 농축 혈소판 혈장(Platelet-Rich Plasma:PRP)의 효과에 대한 실험적 연구

초록

There are so many cases which need grafts of bone defect as the reconstruction of post-tumor resection surgery site or trauma site, surgical management of alveolar cleft and oronasal fistula, bone grafts associated with osseointegrated implants and maxillary sinus lifting procedure. Among the method of bone defect reconstruction, that which uses the fresh autogenous bone has disadvantages that it gives also another trauma on the donor site and volume is reduced after the operation and it has limit of quantity. Another method using the commercial allogenic bone and composite bone like the Hydroxyapatite and Tricalcium phosphate has disadvantages that it takes poor tissue responses and its material works only as the passive scaffold. To gain the functional and much alveolar bone, Research is being carried recently that developing and purifying the various growth factor, applying the results of research to clinical situation. The materials promoting the bone formation are Bone Morphogenetic Protein(BMP), Platelet-Derived Growth Factor(PDGF), Transforming Gruwth Factor-beta(TGF-β). It is reported that Platelet-Rich Plasma(PRP) has PDGF, TGF-β and the abilily of promoting the bone formation. In this study, I compared the defect that have been healed naturally without any bone grafts as the control site, the other defect have been augmented only with bovine bone grafts, with another defect have been augmented with bovine bone grafts and Platelet-Rich Plasma simultaneously. I wanted to see whether much better results of bone formation on using the PRP have been gained, through the method of histologic evaluation. The results was gained as follows on the light microscopic examination. 1. The defect have been augmented with bovine bone grafts and PRP simultaneously showed the higher bone density and much bone formation than that have been augmented only with bovine bone grafts, especially on the eighth week's histologic examination. 2. Comparing the histologic specimen of the defects by the time elapse, Bone grafts with PRP simultaneously had the fastest bone growth rate among the three defects above. 3. The defect that have been heated naturally without any bone grafts had much fibrous tissue and less new bone formation. It seems that using the bone graft and PRP simultaneously on the bone defect gains the better bone healing as the above results.