Bx형 3예에서 ABO유전자형 분석

초록

Background: The knowledge about the nucleotide sequence of the 9th chromosome that regulates the phenotype of the ABO blood group has made ABO genotyping possible. ABO genotyping can play an important role in paternity and forensic investigation. Blood typing of the B subgroup is possible by genotyping the B gene because the B subgroup includes cis AB, which has partially the same genes as the normal B blood group. We evaluated the usefulness of ABO genotyping using the polymerase chain reaction (PCR) method as an alternative test for blood typing in three cases of Bx phenotype. Methods: According to the general blood typing method, which includes cell typing, serum typing, adsorption–elution test, and hemagglutination inhibition test of saliva, we identified the three Bx phenotypes by weak agglutination with anti-A and anti-B serum and the presence of H substance in saliva. Genotyping by PCR method was performed using three primers specific for O allele, B allele, and A/O allele by Fukumori et al. Results: Of the three cases of Bx phenotypes for genotyping, all three cases were typed as BO genotype, showing full agreement with phenotyping. Conclusion: ABO genotyping will be useful as a supplemental and confirmatory test to solve the problem of blood group typing caused by mismatch between cell and serum typings, especially in the B subgroup and when RBCs and saliva are not available for serological phenotyping.

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