원자힘 현미경을 이용한 성장기 흰쥐의 호르몬 결핍이 무릎 연골세포에 미치는 영향 분석

초록

The present study was designed to evaluate the correlation between osteoporosis and osteoarthritis, based on micro-computed tomography (Micro-CT) and atomic force microscopy (AFM). To induce osteoporosis, virgin Sprague-Dawley rats (6 weeks old) were ovariectomized (OVX). All rats were randomly divided in two groups (normal group: 9, OVX group: 6). Knee joint on each rat was scanned by Micro-CT with 18-μm resolution at 0, 4 and 8 weeks. Morphological characteristics were calculated by Micro-CT image analyzer. Knee joint chondrocytes were isolated and immobilized to investigate morphological and mechanical characteristics on AFM measurements. In the OVX group, morphological characteristics significantly changed at all the femur over time(p<0.05). Comparing OVX group to normal group at 8 weeks, morphological characteristics and stiffness of chondrocytes measured by AFM significantly increased in normal group more than OVX group(p<0.05). The results showed that osteoporosis can cause osteoarthritis. The finding of the present study indicated that AFM can analyze the origin of osteoarthritis. In addition, this study is valuable attempt to find the correlation between osteoporosis and osteoarthritis.

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