흥분성 신경전달물질의 Cortical Spreading Depression 유발에 대한 실험적 연구 : 대뇌피질 국소 도포를 위한 새 실험 모형

초록

The purpose of this study was to devise a new model for topical application of chemical substances to rat cerebral cortex that successfully and repeatedly initiated the CSD in conjunction with responses from various excitatory amino acids(EAAs). Various EAA agents, including glutamate itself, that act on single or multiple subtypes of EAA receptor were examined; kainate, aspartate, N-methyl-D-aspartate (NMDA), quisqualate, and alpha-amino-3 -hydroxy- 5-methyl-4-isoxazole-propriate (AMPA). Through the model with a specially designed well buried in 1.5 mm depth of the cerebral cortex, these chemical agents were topically applied to the cortical gray matter. Dose of EAA between 10^(-7) and 10^(-4) M concentration were escalated for triggering the CSD and its rate of consistency in triggering was also evaluated. Total of 50 Sprague-Dawley rats were used and divided into seven groups including the sham group.