타이타늄 표면 거칠기가 골모세포의 이동에 미치는 영향

초록

If Titanium implants can be made truly osteoconductive or made to enhance the rate of osteogenesis, then the problems of treating patients with limited bone mass or the challenge of reducing treatment time can be eliminated. This attempt to identify relevant in vitro cellular measurements that reflect in vivo outcomes of bone formation at implant surfaces is a necessary step toward a rational engineering approach to improving endosseous implant technology. Increased implant surface roughness can increase the bone to implant contact measured in experimental animal models. We hypothesize that one cellular mechanism affecting this process is osteoblast motility or haplotaxis. In this study, an in vitro wounding model using the MG 63 osteosarcoma cell line was used. Titanium discs were prepared from titanium rod and surfaced as either specular polished (S, 0.06 um Ra), machined (M, 0.14 um Ra), grit blasted (G, 0.58 um Ra), or acid etched (A, 0.76 um Ra ). After 24 hours adhesion, a wound (approximately 100 um wide) was created across the lawn or discs were removed and fixed to assess attachment by SEM analysis. After wounding, discs were removed after 0, 24, 48 or 72 hours and fixed for SEM evaluation of motility into the wound. These results indicate that in vitro osteoblast motility can not be correlated with the extent of bone formed at these surfaces in vivo. Alternative in vitro cellular behaviors should be investigated as correlative guides for further dental implant surface engineering.