허혈-재관류시에 골격근에서의 heat shock protein 및 c-fos 유전자 발현에 관한 연구

초록

In this study, we investigated the activity of creatine phosphokinase(CPK) as a marker of damage and the pattern of the expression of heat shock protein(hsp) and c-fos mRNA by in situ hybridization histochemistry in the soleus(red) and gastrocnemius(white) muscles through the ischemia and ischemia-reperfusion processes. The CPK activity showed higher in the ischemia-reperfusion group than the ischemia only group and the longer ischemia before reperfusion increased CPK activity in the ischemia-reperfusion group. The expression of hsp mRNAs peaked at 8hr ischemia in ischemia only group, 4hr reperfusion in 1hr ischemia-reperfusion group and 2hr reperfusion in 2hr ischemia-reperfusion group. The intensity of the hsp mRNAs in ischemia-reperfusion group was stronger than that of ischemia only group. The expression of hsp mRNAs in the gastrocnemius muscle preceded that in the soleus muscle by the same stress. The expression of c-fos mRNAs was relatively high at 8hr ischemia in the gastrocnemius and 12hr ischemia in the soleus muscle but very low in both muscles after 2hr reperfusion in the ischemia-reperfusiion group. From these results, it may be said that the expression of the heat shock protein mRNAs was proportionated to the level of the damage of skeletal muscle and c-fos mRNAs has no specific relation with the expression of hsp mRNAs in the reperfusion damage.

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