Quantitative Estimation of the Total Sulfide Concentration in Live Tissues by Two-Photon Microscopy
DC Field | Value | Language |
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dc.contributor.author | Lim, Chang Su | - |
dc.contributor.author | Das, Sajal Kumar | - |
dc.contributor.author | Yang, Sun Young | - |
dc.contributor.author | Kim, Eun Sun | - |
dc.contributor.author | Chun, Hoon Jai | - |
dc.contributor.author | Cho, Bong Rae | - |
dc.date.available | 2020-11-02T20:42:55Z | - |
dc.date.issued | 2013-10-01 | - |
dc.identifier.issn | 0003-2700 | - |
dc.identifier.issn | 1520-6882 | - |
dc.identifier.uri | https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/10254 | - |
dc.description.abstract | Hydrogen sulfide (H2S) is a newly recognized transmitter, which protects various organs from oxidative stress. In this article, we report a ratiometric two-photon probe, TFCA, which can be excited by 750 nm femtosecond pulses, shows a 110-fold increase in the intensity ratio upon reaction with HS- and high selectivity for HS- and can visualize the total sulfide ([H2S] + [HS-]) distribution in live tissue by two-photon microscopy (TPM). We also developed a kinetic method to quantitatively estimate the total sulfide concentration ([H2S] + [HS-]) in live tissues. The kinetic method allowed us to measure the observed rate constants (k(obs)) for the sulfide-induced deazidation reaction of TFCA in live cells and tissues using TPM. The total sulfide concentration was calculated by using k(obs) = k(2)[HS-], with the k(2) value determined in HEPES/EtOH (1/1, pH = 7.2), and [H2S]/[HS-] = [H+]/K-2. The total sulfide concentration was found to be nearly zero in HeLa cells and 4-7 mu M in rat colon tissues. | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.title | Quantitative Estimation of the Total Sulfide Concentration in Live Tissues by Two-Photon Microscopy | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1021/ac402042k | - |
dc.identifier.scopusid | 2-s2.0-84885008195 | - |
dc.identifier.wosid | 000330017500055 | - |
dc.identifier.bibliographicCitation | ANALYTICAL CHEMISTRY, v.85, no.19, pp 9288 - 9295 | - |
dc.citation.title | ANALYTICAL CHEMISTRY | - |
dc.citation.volume | 85 | - |
dc.citation.number | 19 | - |
dc.citation.startPage | 9288 | - |
dc.citation.endPage | 9295 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.subject.keywordPlus | HYDROGEN-SULFIDE | - |
dc.subject.keywordPlus | FLUORESCENT-PROBES | - |
dc.subject.keywordPlus | RESOLUTION | - |
dc.subject.keywordPlus | MEDIATOR | - |
dc.subject.keywordPlus | COPPER | - |
dc.subject.keywordPlus | ROLES | - |
dc.subject.keywordPlus | H2S | - |
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