Bilirubin attenuates the renal tubular injury by inhibition of oxidative stress and apoptosis
DC Field | Value | Language |
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dc.contributor.author | Oh S.W. | - |
dc.contributor.author | Lee E.S. | - |
dc.contributor.author | Kim S. | - |
dc.contributor.author | Na K.Y. | - |
dc.contributor.author | Chae D.W. | - |
dc.contributor.author | Kim S. | - |
dc.contributor.author | Chin H.J. | - |
dc.date.available | 2020-11-02T22:43:43Z | - |
dc.date.issued | 2013 | - |
dc.identifier.issn | 1471-2369 | - |
dc.identifier.uri | https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/11382 | - |
dc.description.abstract | Background: Bilirubin (BIL) has been recognized as an endogenous antioxidant that shows a protective effect for cardiorenal diseases. We investigated whether administration of BIL had a protective effect on cyclosporine (CsA)-induced nephropathy (CIN), and examined the effects of BIL on the oxidative stress and apoptosis. Methods. BIL was pretreated intraperitoneally three times for a week (60 mg/kg), and CsA was injected for 4 weeks (15 mg/kg/day, subcutaneous). Proximal tubular epithelial (HK2) cells were pretreated with 0.1mg/ml of BIL for 24 hours, and then treated with 20 μM of CsA for another 24 hours. Results: CsA induced marked increases in urine kidney injury molecule-1 (Kim-1) and neutrophil gelatinase-associated lipocalin (NGAL) concentrations (P < 0.05). BIL reduced urine Kim-1 in CIN (P < 0.05), while urine NGAL exhibited a decreasing tendency. In CsA-treated rat kidneys, the protein expression of NOX4 and p22phox was reduced by BIL (P < 0.05). BIL ameliorated CsA-induced arteriolopathy, tubulointerstitial fibrosis, tubular injury, and the apoptosis examined by TUNEL assay (P < 0.01). In HK2 cells, BIL reduced intracellular reactive oxygen species in CsA-treated cells. CsA increased the protein expression of bax, cleaved caspase-9, caspase-3 and the activity of caspase-3; however, the anti-apoptotic bcl-2 protein was reduced. These changes were recovered by BIL (P < 0.05). Conclusions: The direct administration of BIL protected against CsA-induced tubular injury via inhibition of oxidative stress and apoptosis. © 2013 Oh et al.; licensee BioMed Central Ltd. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.title | Bilirubin attenuates the renal tubular injury by inhibition of oxidative stress and apoptosis | - |
dc.type | Article | - |
dc.publisher.location | 영국 | - |
dc.identifier.doi | 10.1186/1471-2369-14-105 | - |
dc.identifier.scopusid | 2-s2.0-84877811017 | - |
dc.identifier.bibliographicCitation | BMC Nephrology, v.14, no.1 | - |
dc.citation.title | BMC Nephrology | - |
dc.citation.volume | 14 | - |
dc.citation.number | 1 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordPlus | bilirubin | - |
dc.subject.keywordPlus | caspase 3 | - |
dc.subject.keywordPlus | caspase 9 | - |
dc.subject.keywordPlus | cyclosporin A | - |
dc.subject.keywordPlus | kidney injury molecule 1 | - |
dc.subject.keywordPlus | neutrophil gelatinase associated lipocalin | - |
dc.subject.keywordPlus | protein bcl 2 | - |
dc.subject.keywordPlus | reactive oxygen metabolite | - |
dc.subject.keywordPlus | animal experiment | - |
dc.subject.keywordPlus | animal model | - |
dc.subject.keywordPlus | animal tissue | - |
dc.subject.keywordPlus | apoptosis | - |
dc.subject.keywordPlus | article | - |
dc.subject.keywordPlus | controlled study | - |
dc.subject.keywordPlus | human | - |
dc.subject.keywordPlus | human cell | - |
dc.subject.keywordPlus | kidney epithelium | - |
dc.subject.keywordPlus | kidney tubule damage | - |
dc.subject.keywordPlus | male | - |
dc.subject.keywordPlus | nick end labeling | - |
dc.subject.keywordPlus | nonhuman | - |
dc.subject.keywordPlus | oxidative stress | - |
dc.subject.keywordPlus | protein expression | - |
dc.subject.keywordPlus | rat | - |
dc.subject.keywordPlus | Acute Kidney Injury | - |
dc.subject.keywordPlus | Animals | - |
dc.subject.keywordPlus | Apoptosis | - |
dc.subject.keywordPlus | Bilirubin | - |
dc.subject.keywordPlus | Cell Line | - |
dc.subject.keywordPlus | Humans | - |
dc.subject.keywordPlus | Injections, Intraperitoneal | - |
dc.subject.keywordPlus | Kidney Tubules | - |
dc.subject.keywordPlus | Male | - |
dc.subject.keywordPlus | Oxidative Stress | - |
dc.subject.keywordPlus | Rats | - |
dc.subject.keywordPlus | Rats, Sprague-Dawley | - |
dc.subject.keywordAuthor | Apoptosis | - |
dc.subject.keywordAuthor | Bilirubin | - |
dc.subject.keywordAuthor | Cyclosporine | - |
dc.subject.keywordAuthor | Oxidative stress | - |
dc.subject.keywordAuthor | Renal injury | - |
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