Evaluation of Sofia fluorescent immunoassay analyzer for influenza A/B virus
DC Field | Value | Language |
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dc.contributor.author | Lee, Chang Kyu | - |
dc.contributor.author | Cho, Chi Hyun | - |
dc.contributor.author | Woo, Mi Kyung | - |
dc.contributor.author | Nyeck, Agnes E. | - |
dc.contributor.author | Lim, Chae Seung | - |
dc.contributor.author | Kim, Woo Joo | - |
dc.date.available | 2020-11-02T22:46:12Z | - |
dc.date.issued | 2012-11 | - |
dc.identifier.issn | 1386-6532 | - |
dc.identifier.issn | 1873-5967 | - |
dc.identifier.uri | https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/11602 | - |
dc.description.abstract | Background: The influenza virus causes seasonal epidemics which are associated with high morbidity and mortality. Rapid diagnostics tests (RDT) are frequently used to make a quick influenza diagnosis to confirm the clinical suspicion, despite their low sensitivity. Objectives: Assess the performance of the Sofia Influenza A + B Fluorescence Immunoassay (Quidel, San Diego, CA). Study design: Nasopharyngeal swabs, taken from 241 patients (influenza A (n = 73)/B (n = 72), negative samples (n = 96)) were analyzed using the Sofia Influenza A + B Fluorescence Immunoassay, BinaxNOW Influenza A/B antigen kit (Alere Inc., USA), Directigen EZ Flu A and B (Becton Dickinson, USA), real-time RT-PCR and an influenza virus culture. Results: There was a significant difference between the performance of rapid antigen tests and the Sofia FIA, when compared to the RT-PCR, in the detection of influenza strain A and B. Indeed, the Sofia FIA displayed sensitivities of 82.2% and 77.8% for strains A and B respectively, whereas sensitivities of BinaxNOW Influenza A/B antigen kit, and Directigen Flu A and B were 54.8%, and 68.5% for influenza A, and 62.5%, and 52.8% for influenza B respectively. The average RT-PCR threshold cycle (C-t) (+/- SD) for the Sofia Influenza A + B Fluorescence Immunoassay-positive specimens was higher than those of the BinaxNOW Influenza A/B antigen and the Directigen EZ Flu A and B kit positive specimens. Conclusion: Compared to other RDTs, the Sofia Influenza A + B Fluorescence Immunoassay is a sensitive, and rapid method for the detection and discrimination between influenza A and B. (C) 2012 Elsevier B.V. All rights reserved. | - |
dc.format.extent | 5 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ELSEVIER | - |
dc.title | Evaluation of Sofia fluorescent immunoassay analyzer for influenza A/B virus | - |
dc.type | Article | - |
dc.publisher.location | 네델란드 | - |
dc.identifier.doi | 10.1016/j.jcv.2012.07.008 | - |
dc.identifier.scopusid | 2-s2.0-84866402457 | - |
dc.identifier.wosid | 000309113000009 | - |
dc.identifier.bibliographicCitation | JOURNAL OF CLINICAL VIROLOGY, v.55, no.3, pp 239 - 243 | - |
dc.citation.title | JOURNAL OF CLINICAL VIROLOGY | - |
dc.citation.volume | 55 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 239 | - |
dc.citation.endPage | 243 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Virology | - |
dc.relation.journalWebOfScienceCategory | Virology | - |
dc.subject.keywordPlus | RAPID DETECTION | - |
dc.subject.keywordPlus | TESTS | - |
dc.subject.keywordPlus | SENSITIVITY | - |
dc.subject.keywordPlus | PERFORMANCE | - |
dc.subject.keywordPlus | DIAGNOSIS | - |
dc.subject.keywordPlus | ACCURACY | - |
dc.subject.keywordPlus | CHILDREN | - |
dc.subject.keywordAuthor | Influenza | - |
dc.subject.keywordAuthor | Virus culture | - |
dc.subject.keywordAuthor | Fluorescent immunoassay | - |
dc.subject.keywordAuthor | Rapid diagnostic test | - |
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