Reactive oxygen species enhance differentiation of human embryonic stem cells into mesendodermal lineage
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ji A.-R. | - |
dc.contributor.author | Ku S.-Y. | - |
dc.contributor.author | Cho M.S. | - |
dc.contributor.author | Kim Y.Y. | - |
dc.contributor.author | Kim Y.J. | - |
dc.contributor.author | Oh S.K. | - |
dc.contributor.author | Kim S.H. | - |
dc.contributor.author | Moon S.Y. | - |
dc.contributor.author | Choi Y.M. | - |
dc.date.available | 2020-11-03T07:44:09Z | - |
dc.date.issued | 2010-03-31 | - |
dc.identifier.issn | 1226-3613 | - |
dc.identifier.issn | 2092-6413 | - |
dc.identifier.uri | https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/15456 | - |
dc.description.abstract | Recently, reactive oxygen species (ROS) have been studied as a regulator of differentiation into specific cell types in embryonic stem cells (ESCs). However, ROS role in human ESCs (hESCs) is unknown because mouse ESCs have been used mainly for most studies. Herein we suggest that ROS generation may play a critical role in differentiation of hESCs; ROS enhances differentiation of hESCs into bi-potent mesendodermal cell lineage via ROS-involved signaling pathways. In ROS-inducing conditions, expression of pluripotency markers (Oct4, Tra 1-60, Nanog, and Sox2) of hESCs was decreased, while expression of mesodermal and endodermal markers was increased. Moreover, these differentiation events of hESCs in ROS-inducing conditions were decreased by free radical scavenger treatment. hESC-derived embryoid bodies (EBs) also showed similar differentiation patterns by ROS induction. In ROS-related signaling pathway, some of the MAPKs family members in hESCs were also affected by ROS induction. p38 MAPK and AKT (protein kinases B, PKB) were inactivated significantly by buthionine sulfoximine (BSO) treatment. JNK and ERK phosphorylation levels were increased at early time of BSO treatment but not at late time point. Moreover, MAPKs family-specific inhibitors could prevent the mesendodermal differentiation of hESCs by ROS induction. Our results demonstrate that stemness and differentiation of hESCs can be regulated by environmental factors such as ROS. | - |
dc.format.extent | 12 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Nature Publishing Group | - |
dc.title | Reactive oxygen species enhance differentiation of human embryonic stem cells into mesendodermal lineage | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.3858/emm.2010.42.3.018 | - |
dc.identifier.scopusid | 2-s2.0-77950523031 | - |
dc.identifier.wosid | 000276279800003 | - |
dc.identifier.bibliographicCitation | Experimental and Molecular Medicine, v.42, no.3, pp 175 - 186 | - |
dc.citation.title | Experimental and Molecular Medicine | - |
dc.citation.volume | 42 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 175 | - |
dc.citation.endPage | 186 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.subject.keywordPlus | buthionine sulfoximine | - |
dc.subject.keywordPlus | hepatocyte nuclear factor 3beta | - |
dc.subject.keywordPlus | Janus kinase | - |
dc.subject.keywordPlus | mitogen activated protein kinase | - |
dc.subject.keywordPlus | mitogen activated protein kinase p38 | - |
dc.subject.keywordPlus | octamer transcription factor 4 | - |
dc.subject.keywordPlus | protein kinase B | - |
dc.subject.keywordPlus | reactive oxygen metabolite | - |
dc.subject.keywordPlus | transcription factor NANOG | - |
dc.subject.keywordPlus | transcription factor Nkx2.2 | - |
dc.subject.keywordPlus | transcription factor PDX 1 | - |
dc.subject.keywordPlus | transcription factor Sox17 | - |
dc.subject.keywordPlus | transcription factor Sox2 | - |
dc.subject.keywordPlus | biological marker | - |
dc.subject.keywordPlus | mitogen activated protein kinase | - |
dc.subject.keywordPlus | reactive oxygen metabolite | - |
dc.subject.keywordPlus | scavenger | - |
dc.subject.keywordPlus | apoptosis | - |
dc.subject.keywordPlus | article | - |
dc.subject.keywordPlus | cell culture | - |
dc.subject.keywordPlus | cell differentiation | - |
dc.subject.keywordPlus | cell lineage | - |
dc.subject.keywordPlus | dose response | - |
dc.subject.keywordPlus | down regulation | - |
dc.subject.keywordPlus | embryonic stem cell | - |
dc.subject.keywordPlus | endoderm | - |
dc.subject.keywordPlus | environmental factor | - |
dc.subject.keywordPlus | enzyme activation | - |
dc.subject.keywordPlus | enzyme inactivation | - |
dc.subject.keywordPlus | enzyme induction | - |
dc.subject.keywordPlus | gene expression | - |
dc.subject.keywordPlus | human | - |
dc.subject.keywordPlus | human cell | - |
dc.subject.keywordPlus | in vitro study | - |
dc.subject.keywordPlus | mesoderm | - |
dc.subject.keywordPlus | phase contrast microscopy | - |
dc.subject.keywordPlus | pluripotent stem cell | - |
dc.subject.keywordPlus | protein expression | - |
dc.subject.keywordPlus | protein function | - |
dc.subject.keywordPlus | protein phosphorylation | - |
dc.subject.keywordPlus | reverse transcription polymerase chain reaction | - |
dc.subject.keywordPlus | signal transduction | - |
dc.subject.keywordPlus | upregulation | - |
dc.subject.keywordPlus | animal embryo | - |
dc.subject.keywordPlus | cell line | - |
dc.subject.keywordPlus | cytology | - |
dc.subject.keywordPlus | drug effects | - |
dc.subject.keywordPlus | embryonic stem cell | - |
dc.subject.keywordPlus | enzymology | - |
dc.subject.keywordPlus | metabolism | - |
dc.subject.keywordPlus | Biological Markers | - |
dc.subject.keywordPlus | Cell Differentiation | - |
dc.subject.keywordPlus | Cell Line | - |
dc.subject.keywordPlus | Cell Lineage | - |
dc.subject.keywordPlus | Cells, Cultured | - |
dc.subject.keywordPlus | Down-Regulation | - |
dc.subject.keywordPlus | Embryo, Mammalian | - |
dc.subject.keywordPlus | Embryonic Stem Cells | - |
dc.subject.keywordPlus | Endoderm | - |
dc.subject.keywordPlus | Enzyme Activation | - |
dc.subject.keywordPlus | Free Radical Scavengers | - |
dc.subject.keywordPlus | Humans | - |
dc.subject.keywordPlus | Mesoderm | - |
dc.subject.keywordPlus | Mitogen-Activated Protein Kinases | - |
dc.subject.keywordPlus | Pluripotent Stem Cells | - |
dc.subject.keywordPlus | Reactive Oxygen Species | - |
dc.subject.keywordPlus | Up-Regulation | - |
dc.subject.keywordAuthor | Cell differentiation | - |
dc.subject.keywordAuthor | Embryonic stem cells | - |
dc.subject.keywordAuthor | Mitogen-activated protein kinases | - |
dc.subject.keywordAuthor | Reactive oxygen species | - |
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