In vitro response of primary human bone marrow stromal cells to recombinant human bone morphogenic protein-2 in the early and late stages of osteoblast differentiation
DC Field | Value | Language |
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dc.contributor.author | Kim, In Sook | - |
dc.contributor.author | Song, Yoon Mi | - |
dc.contributor.author | Cho, Tae Hyung | - |
dc.contributor.author | Park, Yong Doo | - |
dc.contributor.author | Lee, Kyu Back | - |
dc.contributor.author | Noh, Insup | - |
dc.contributor.author | Weber, Franz | - |
dc.contributor.author | Hwang, Soon Jung | - |
dc.date.available | 2020-11-03T09:49:23Z | - |
dc.date.issued | 2008-09 | - |
dc.identifier.issn | 0012-1592 | - |
dc.identifier.issn | 1440-169X | - |
dc.identifier.uri | https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/16825 | - |
dc.description.abstract | A number of factors must be added to human bone marrow stromal cells (hBMSCs) in vitro to induce osteogenesis, including ascorbic acid (AA), beta-glycerophosphate (GP), and dexamethasone (Dex). Bone morphogenic protein (BMP)-2 is an osteoinductive factor that can commit stromal cells to differentiate into osteoblasts. However, it is still not clear whether the addition of BMP-2 alone in vitro can induce hBMSCs to complete osteoblast differentiation, resulting in matrix mineralization. This study compares the effects of BMP-2 and Dex, alone and combined, on the early and late stages of hBMSC differentiation. We found that BMP-2 causes a significant induction of alkaline phosphatase (ALP) activity in hBMSCs, with a transcriptional upregulation of known BMP-2-responsive genes, and the stable expression of cbfa1 in the nucleus and the regions surrounding the nucleus in the early phase of osteoblast differentiation. However, continuous treatment with BMP-2 alone at doses ranging from 100 to 300 ng/mL results in a less efficient enhancement of in vitro matrix mineralization, despite a significant induction of ALP activity at a concentration of 100 ng/mL. Our results reflect how the effects of BMP-2 on hBMSCs can vary depending on the stage of osteoblast differentiation, and highlight the need to understand the role of BMP-2 in primary hBMSCs derived from diverse sources in order to increase the efficiency of using BMP-2 in osteoinductive therapies. | - |
dc.format.extent | 12 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | WILEY | - |
dc.title | In vitro response of primary human bone marrow stromal cells to recombinant human bone morphogenic protein-2 in the early and late stages of osteoblast differentiation | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1111/j.1440-169X.2008.01052.x | - |
dc.identifier.scopusid | 2-s2.0-49349107314 | - |
dc.identifier.wosid | 000258287000003 | - |
dc.identifier.bibliographicCitation | DEVELOPMENT GROWTH & DIFFERENTIATION, v.50, no.7, pp 553 - 564 | - |
dc.citation.title | DEVELOPMENT GROWTH & DIFFERENTIATION | - |
dc.citation.volume | 50 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | 553 | - |
dc.citation.endPage | 564 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Developmental Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Developmental Biology | - |
dc.subject.keywordPlus | MESENCHYMAL STEM-CELLS | - |
dc.subject.keywordPlus | ALKALINE-PHOSPHATASE | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | BETA-CATENIN | - |
dc.subject.keywordPlus | BMP-2 | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | TRANSDIFFERENTIATION | - |
dc.subject.keywordPlus | RUNX2/CBFA1 | - |
dc.subject.keywordPlus | REGULATORS | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.subject.keywordAuthor | alkaline phosphatase | - |
dc.subject.keywordAuthor | bone morphogenic protein-2 | - |
dc.subject.keywordAuthor | human bone marrow stromal cells | - |
dc.subject.keywordAuthor | in vitro matrix mineralization | - |
dc.subject.keywordAuthor | osteoblast differentiation | - |
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