A genotype-independent real-time PCR assay for quantification of hepatitis B virus DNA
DC Field | Value | Language |
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dc.contributor.author | Liu Y. | - |
dc.contributor.author | Hussain M. | - |
dc.contributor.author | Wong S. | - |
dc.contributor.author | Fung S.K. | - |
dc.contributor.author | Yim H.J. | - |
dc.contributor.author | Lok A.S.F. | - |
dc.date.available | 2020-11-03T12:51:44Z | - |
dc.date.issued | 2007 | - |
dc.identifier.issn | 0095-1137 | - |
dc.identifier.issn | 1098-660X | - |
dc.identifier.uri | https://scholarworks.korea.ac.kr/kumedicine/handle/2020.sw.kumedicine/18404 | - |
dc.description.abstract | Accurate quantification of hepatitis B virus (HBV) DNA levels is important for monitoring patients with chronic HBV infection and for assessing their responses to antiviral therapy. This study aimed to develop a real-time PCR assay that is sensitive and can accurately quantify a wide range of HBV DNA levels across the known HBV genotypes. An in-house real-time PCR assay using primers and a TaqMan probe in a highly conserved region of the HBV surface gene was designed. The assay was standardized against a WHO standard and validated against plasmids of HBV genotypes A through H. The linear quantification range was approximately 5 × 100 to 2.0 × 109 IU/ml. Results of samples from patients infected with HBV genotypes A through H tested using our real-time in-house PCR assay showed an excellent correlation with those of the Cobas Amplicor HBV Monitor (R2 = 0.9435) and the Cobas TaqMan HBV (R2 = 0.9873) tests. We have established a real-time PCR assay that is genotype independent and can accurately quantify a wide range of HBV DNA levels. Further studies of additional samples are ongoing to validate the genotype independence of our assay. Copyright © 2007, American Society for Microbiology. All Rights Reserved. | - |
dc.format.extent | 6 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.title | A genotype-independent real-time PCR assay for quantification of hepatitis B virus DNA | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1128/JCM.00709-06 | - |
dc.identifier.scopusid | 2-s2.0-33847048970 | - |
dc.identifier.bibliographicCitation | Journal of Clinical Microbiology, v.45, no.2, pp 553 - 558 | - |
dc.citation.title | Journal of Clinical Microbiology | - |
dc.citation.volume | 45 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 553 | - |
dc.citation.endPage | 558 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordPlus | virus DNA | - |
dc.subject.keywordPlus | accuracy | - |
dc.subject.keywordPlus | article | - |
dc.subject.keywordPlus | assay | - |
dc.subject.keywordPlus | controlled study | - |
dc.subject.keywordPlus | correlation analysis | - |
dc.subject.keywordPlus | DNA determination | - |
dc.subject.keywordPlus | genotype | - |
dc.subject.keywordPlus | Hepatitis B virus | - |
dc.subject.keywordPlus | human | - |
dc.subject.keywordPlus | human tissue | - |
dc.subject.keywordPlus | intermethod comparison | - |
dc.subject.keywordPlus | molecular probe | - |
dc.subject.keywordPlus | nonhuman | - |
dc.subject.keywordPlus | nucleotide sequence | - |
dc.subject.keywordPlus | plasmid | - |
dc.subject.keywordPlus | priority journal | - |
dc.subject.keywordPlus | real time polymerase chain reaction | - |
dc.subject.keywordPlus | validation process | - |
dc.subject.keywordPlus | DNA Primers | - |
dc.subject.keywordPlus | DNA, Viral | - |
dc.subject.keywordPlus | Genotype | - |
dc.subject.keywordPlus | Hepatitis B | - |
dc.subject.keywordPlus | Hepatitis B virus | - |
dc.subject.keywordPlus | Humans | - |
dc.subject.keywordPlus | Polymerase Chain Reaction | - |
dc.subject.keywordPlus | Reagent Kits, Diagnostic | - |
dc.subject.keywordPlus | Sensitivity and Specificity | - |
dc.subject.keywordPlus | Taq Polymerase | - |
dc.subject.keywordPlus | Hepatitis B virus | - |
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